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Medientyp:
E-Artikel
Titel:
Direct Excitation of Hypocretin/Orexin Cells by Extracellular ATP at P2X Receptors
Beteiligte:
Wollmann, Guido;
Acuna-Goycolea, Claudio;
van den Pol, Anthony N.
Erschienen:
American Physiological Society, 2005
Erschienen in:
Journal of Neurophysiology, 94 (2005) 3, Seite 2195-2206
Sprache:
Englisch
DOI:
10.1152/jn.00035.2005
ISSN:
0022-3077;
1522-1598
Entstehung:
Anmerkungen:
Beschreibung:
Hypocretin/orexin (hcrt) neurons play an important role in hypothalamic arousal and energy homeostasis. ATP may be released by neurons or glia or by pathological conditions. Here we studied the effect of extracellular ATP on hypocretin cells using whole cell patch-clamp recording in hypothalamic slices of transgenic mice expressing green fluorescent protein (GFP) exclusively in hcrt-producing cells. Local application of ATP induced a dose-dependent increase in spike frequency. In the presence of TTX, ATP (100 μM) depolarized the cells by 7.8 ± 1.2 mV. In voltage clamp under blockade of synaptic activity with the GABAA receptor antagonist bicuculline, and ionotropic glutamate receptor antagonists dl-2-amino-5-phosphonopentanoic acid (AP-5) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), ATP (100 μM) evoked an 18 pA inward current. The inward current was blocked by extracellular choline substitution for Na+, had a reversal potential of −27 mV, and was not affected by nominally Ca2+-free external buffer, suggesting that ATP activated a nonselective cation current. All excitatory effects of ATP showed rapid attenuation. ATP-induced excitatory actions were mimicked by nonhydrolyzable ATP-γ-S but not by α,β-MeATP and inhibited by the purinoceptor antagonists suramin and pyridoxal phosphate-6-azo(benzene-2,4-disulfonic acid) tetrasodium salt (PPADS). The current was potentiated by a decrease in bath pH, suggesting P2X2 subunit involvement. Frequency and amplitude of spontaneous and miniature synaptic events were not altered by ATP. Suramin, but not PPADS, caused a small suppression of evoked excitatory synaptic potentials. Together, these results show a depolarizing response to extracellular ATP that would lead to an increased activity of the hypocretin arousal system.