Erschienen in:
Evidence-Based Complementary and Alternative Medicine, 2014 (2014) 1
Sprache:
Englisch
DOI:
10.1155/2014/429256
ISSN:
1741-427X;
1741-4288
Entstehung:
Anmerkungen:
Beschreibung:
Kalanchoe pinnata (KP) is popularly used for treating inflammatory diseases. This study investigated the antinociceptive, antiedematogenic, and anti‐inflammatory potential of the subcutaneous administration of KP flower aqueous extract (KPFE), its ethyl acetate (EtOAcF) and butanol (BuOHF) fractions, and the main KP flavonoid [quercetin 3‐O‐α‐L‐arabinopyranosyl (1 → 2) α‐L‐rhamnopyranoside] (KPFV) in mice, as well as its possible mechanisms of action. KPFE (30–300 mg/kg) and KPFV (1–10 mg/kg) inhibited the acetic acid‐induced writhing (ID50 = 164.8 and 9.4 mg/kg, resp.). KPFE (300 mg/kg), EtOAcF (12 mg/kg), BuOHF (15 mg/kg), or KPFV (0.3–3.0 mg/kg) reduced leukocyte migration on carrageenan‐induced pleurisy (ID50 = 2.0 mg/kg for KPFV). KPFE (3–30 mg/kg) and KPFV (0.3–3.0 mg/kg) reduced the croton oil‐induced ear edema (ID50 = 4.3 and 0.76 mg/kg, resp.). KPFE and KPFV reduced the TNF‐α concentration in the pleural exudates on carrageenan‐induced pleurisy test. Moreover, KPFV inhibited COX‐1 (IC50 = 22.1 μg/mL) and COX‐2 (IC50 > 50 μg/mL). The selectivity index (COX‐/COX‐) was <0.44. These results indicate that KPFE and KPFV produced antinociceptive, antiedematogenic, and anti‐inflammatory activities through COX inhibition and TNF‐α reduction, revealing that the main flavonoid in KP flowers and leaves plays an important role in the ethnomedicinal use of the plant.