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Medientyp:
E-Artikel
Titel:
How Neutrophil Extracellular Traps Become Visible
Beteiligte:
de Buhr, Nicole;
von Köckritz-Blickwede, Maren
Erschienen:
Hindawi Limited, 2016
Erschienen in:
Journal of Immunology Research, 2016 (2016), Seite 1-13
Sprache:
Englisch
DOI:
10.1155/2016/4604713
ISSN:
2314-7156;
2314-8861
Entstehung:
Anmerkungen:
Beschreibung:
Neutrophil extracellular traps (NETs) have been identified as a fundamental innate immune defense mechanism against different pathogens. NETs are characterized as released nuclear DNA associated with histones and granule proteins, which form an extracellular web-like structure that is able to entrap and occasionally kill certain microbes. Furthermore, NETs have been shown to contribute to several noninfectious disease conditions when released by activated neutrophils during inflammation. The identification of NETs has mainly been succeeded by various microscopy techniques, for example, immunofluorescence microscopy, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Since the last years the development and improvement of new immunofluorescence-based techniques enabled optimized visualization and quantification of NETs. On the one handin vitrolive-cell imaging led to profound new ideas about the mechanisms involved in the formation and functionality of NETs. On the other hand different intravital,in vivo, andin situmicroscopy techniques led to deeper insights into the role of NET formation during health and disease. This paper presents an overview of the main used microscopy techniques to visualize NETs and describes their advantages as well as disadvantages.