Beschreibung:
<jats:title>Abstract</jats:title>
<jats:p>The KAI1 tetraspanin also known as CD82 is a widely expressed cell surface molecule involved in organizing many diverse cellular signaling processes. Importantly KAI1/CD82 was shown to suppress metastasis but not tumorigenicity in animal models of metastasis, establishing KAI1/CD82 as one of a class of metastasis suppressor genes. The function of KAI1/CD82 regarding its role in controlling metastasis is under scrutiny and many questions remain as to its function in regulating this and other cellular processes. In order to further assess the functions of KAI1/CD82 we examined the effect of germline deletion on viability, fertility, lymphocyte composition, blood chemistry and performed a detailed pathologic analysis of tissues in Kai1/CD82 deficient mice and their wild-type and heterozygote littermates. Interestingly mice deficient in KAI1/CD82 showed no obvious genotype associated defects in any of these processes as compared to their wild-type littermates and displayed no genotype associated abnormalities after 12 or 18 months of life. To further explore the lack of noticeable phenotypes in these mice we employed expression profiling of non-immortal MEF cultures to assess the effect of KAI1/CD82 on global gene expression. Gene expression of MEFs indicated distinct sex specific and genotype specific profiles suggesting some genes as compensatory candidates which might explain the lack of appreciable phenotypes in KAI1/CD82 deficient mice.</jats:p>
<jats:p>Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3375.</jats:p>