Eichinger, Sabine;
Wolzt, Michael;
Schneider, Barbara;
Nieszpaur-Los, Malgorzata;
Heinrichs, Hubert;
Lechner, Klaus;
Eichler, Hans-Georg;
Kyrle, Paul A.
Effects of Recombinant Hirudin (r-Hirudin, HBW 023) on Coagulation and Platelet Activation In Vivo : Comparison With Unfractionated Heparin and a Low-Molecular-Weight Heparin Preparation (Fragmin)
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Medientyp:
E-Artikel
Titel:
Effects of Recombinant Hirudin (r-Hirudin, HBW 023) on Coagulation and Platelet Activation In Vivo : Comparison With Unfractionated Heparin and a Low-Molecular-Weight Heparin Preparation (Fragmin)
:
Comparison With Unfractionated Heparin and a Low-Molecular-Weight Heparin Preparation (Fragmin)
Beteiligte:
Eichinger, Sabine;
Wolzt, Michael;
Schneider, Barbara;
Nieszpaur-Los, Malgorzata;
Heinrichs, Hubert;
Lechner, Klaus;
Eichler, Hans-Georg;
Kyrle, Paul A.
Beschreibung:
Abstract In a double-blind, randomized, crossover study, we investigated in 15 healthy male volunteers the effects of recombinant (r-) hirudin (HBW 023, 0.35 mg/kg body wt SC), unfractionated heparin (UFH, HeparinNovo; 150 IU/kg body wt SC), and a low-molecular-weight heparin preparation (LMWH, Fragmin; 75 IU/kg body wt SC) on coagulation and platelet activation in vivo by measuring specific coagulation-activation peptides (prothrombin fragment 1+2 [F1+2], thrombin–antithrombin-III complex [TAT], and β-thromboglobulin [β-TG]) in bleeding-time blood (activated state) and venous blood (basal state). In bleeding-time blood, r-hirudin and the heparin preparations significantly inhibited formation of both TAT and F1+2. However, the inhibitory effect of r-hirudin on F1+2 generation was short-lived and weaker compared with that of UFH and LMWH, and the TAT-to-F1+2 ratio was significantly lower after r-hirudin than after UFH or LMWH. Thus, in vivo, when the coagulation system is in an activated state, r-hirudin exerts its anticoagulant effects predominantly by inhibiting thrombin (factor IIa), whereas UFH and LMWH are directed against both factors Xa and IIa. A different mode of action for UFH and LMWH was not detectable. In venous blood, r-hirudin caused a moderate reduction in TAT formation and an increase (at 1 hour) rather than a decrease in F1+2 generation. Formation of TAT and F1+2 was suppressed at various time points following both UFH and LMWH. There was no difference in the TAT-to-F1+2 ratio after r-hirudin and heparin. Thus, a predominant effect of r-hirudin on factor IIa (as found in bleeding-time blood) was not detectable in venous blood. In bleeding-time blood, r-hirudin (but neither UFH nor LMWH) significantly inhibited β-TG release. In contrast, both UFH and LMWH caused an increase in β-TG 10 hours after heparin administration. Our observation of reduced platelet function after r-hirudin compared with delayed platelet activation following UFH and LMWH suggests an advantage of r-hirudin over heparin, especially in those clinical situations (such as arterial thromboembolism) where enhanced platelet activity has been shown to be of particular importance.