Franceschi, Francesco;
Sepulveda, Antonia R.;
Gasbarrini, Antonio;
Pola, Paolo;
Silveri, Nicolò Gentiloni;
Gasbarrini, Giovanni;
Graham, David Y.;
Genta, Robert M.
Cross-Reactivity of Anti-CagA Antibodies With Vascular Wall Antigens : Possible Pathogenic Link Between Helicobacter pylori Infection and Atherosclerosis
Possible Pathogenic Link Between
<i>Helicobacter pylori</i>
Infection and Atherosclerosis
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Medientyp:
E-Artikel
Titel:
Cross-Reactivity of Anti-CagA Antibodies With Vascular Wall Antigens : Possible Pathogenic Link Between Helicobacter pylori Infection and Atherosclerosis
:
Possible Pathogenic Link Between
<i>Helicobacter pylori</i>
Infection and Atherosclerosis
Beteiligte:
Franceschi, Francesco;
Sepulveda, Antonia R.;
Gasbarrini, Antonio;
Pola, Paolo;
Silveri, Nicolò Gentiloni;
Gasbarrini, Giovanni;
Graham, David Y.;
Genta, Robert M.
Beschreibung:
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<jats:italic>Background</jats:italic>
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<jats:italic>Helicobacter pylori</jats:italic>
-CagA positive strains have been shown to be associated with atherosclerosis. However, the pathogenesis is still undetermined. The aim of this study was to determine whether anti-CagA antibodies cross-react with antigens of normal and atherosclerotic arteries.
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<jats:italic>Methods and Results</jats:italic>
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Eight umbilical cord sections, 14 atherosclerotic artery sections, and 10 gastrointestinal tract sections were examined by immunohistochemistry using polyclonal anti-CagA antibodies. Five atherosclerotic and 3 normal artery samples were also lysed in ice-cold lysis buffer containing protease inhibitors and were immunoprecipitated using the same antibodies. Anti-CagA antibodies reacted with cytoplasm and nuclei of smooth muscle cells in umbilical cord and atherosclerotic vessel sections, cytoplasm of fibroblasts-like cells in intimal atherosclerotic plaques, and the cell membranes of endothelial cells. Anti-CagA antibodies also specifically immunoprecipitated 2 high molecular weight antigens of 160 and 180 kDa from both normal and atherosclerotic artery lysates.
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<jats:italic>Conclusions</jats:italic>
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Anti-CagA antibodies cross-react with antigens of both normal and atherosclerotic blood vessels. We speculate that the binding of anti-CagA antibodies to those antigens in injured arteries could influence the progression of atherosclerosis in CagA-positive
<jats:italic>H pylori</jats:italic>
-infected patients.
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