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Melenovsky, Vojtech; Petrak, Jiri; Mracek, Tomas; Benes, Jan; Drahota, Zdenek; Pluhacek, Tomas; Nuskova, Hana; Jana, Kovalcikova; Pirk, Jan; Houstek, Josef

Abstract 17165: Myocardial Iron Depletion In Patients With Heart Failure Is Associated With Mitochondrial Dysfunction And Attenuation Of Citrate Synthase Activity

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  • Medientyp: E-Artikel
  • Titel: Abstract 17165: Myocardial Iron Depletion In Patients With Heart Failure Is Associated With Mitochondrial Dysfunction And Attenuation Of Citrate Synthase Activity
  • Beteiligte: Melenovsky, Vojtech; Petrak, Jiri; Mracek, Tomas; Benes, Jan; Drahota, Zdenek; Pluhacek, Tomas; Nuskova, Hana; Jana, Kovalcikova; Pirk, Jan; Houstek, Josef
  • Erschienen: Ovid Technologies (Wolters Kluwer Health), 2015
  • Erschienen in: Circulation
  • Sprache: Englisch
  • DOI: 10.1161/circ.132.suppl_3.17165
  • ISSN: 1524-4539; 0009-7322
  • Schlagwörter: Physiology (medical) ; Cardiology and Cardiovascular Medicine
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p> <jats:bold>Introduction:</jats:bold> Very little is known about the determinants and consequences of abnormal iron (Fe) content in the failing human myocardium. </jats:p> <jats:p> <jats:bold>Hypothesis:</jats:bold> We hypothesized that myocardial Fe deficiency (ID) in heart failure (HF) is associated with impaired mitochondrial function of Fe-regulated enzymes of respiratory chain (RC) and Krebs (TCA) cycle. </jats:p> <jats:p> <jats:bold>Methods:</jats:bold> LV samples were obtained from 90 consecutive patients undergoing transplantation (HF: LVEF 23±8%, age 53±11y, 83% males, 46% CAD, 24 % diabetes) and from 38 HF-free organ donors (CON: LVEF 57±12%, age 42±15y, 50% males, 14% with diabetes). Activities of citrate synthase (CS, Krebs cycle) and RC enzymes NADH-cytochrome c oxidoreductase (NCCR), succinate cytochrome c oxidoreductase (SCCR) and cytochrome c oxidase (COX) were measured in homogenates. Total Fe was measured by ICP-MS after microwave-assisted digestion of lyophilized samples. </jats:p> <jats:p> <jats:bold>Results:</jats:bold> Compared to CON, HF patients had lower total myocardial Fe (156±41 vs 200±38 μg/g dry weight, p&lt;0.001). Myocardial ID (Fe&lt;124.8 μg/g; 2*SD from the mean of CON) was present in 22 % of HF patients. HF+ID patients had more often diabetes (45% vs 18%, p=0.02), but similar age, gender, renal function, haemoglobin concentration, HF aetiology, LVEF or BNP as non-ID HF. Activities of CS, COX, SCCR and NCCR were all reduced in HF vs. CON (-21%, -34%, -28% and -26%, all p&lt;0.001). In HF, Fe correlated with CS (r=0.30, p=0.004) and SCCR activities (r=0.24, p=0.02), but not with COX or NCCR. In pooled cohort, Fe also displayed strongest relation with CS (figure). HF+ID patients displayed reduced CS activity (516±145 vs 613±19, p=0.03), in contrast to preserved RC enzymes. </jats:p> <jats:p> <jats:bold>Conclusions:</jats:bold> Myocardial Fe content is systematically diminished in advanced HF and is associated with mitochondrial dysfunction, in particular with reduced CS activity. These relations may reflect Fe-dependent regulation of aconitase (TCA cycle enzyme) and may influence the utilization of energetic substrates. </jats:p> <jats:p> <jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" orientation="portrait" position="float" xlink:href="g17165.jpeg" /> </jats:p>
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