• Medientyp: E-Artikel
  • Titel: A Robust Procedure for the Automated Measurement of Total Serum Bilirubin Using Potassium Ferricyanide
  • Beteiligte: O'Leary, Niall; Pembroke, A; Duggan, P F
  • Erschienen: SAGE Publications, 1993
  • Erschienen in: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
  • Sprache: Englisch
  • DOI: 10.1177/000456329303000211
  • ISSN: 0004-5632; 1758-1001
  • Schlagwörter: Clinical Biochemistry ; General Medicine
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p> Bilirubin is oxidized to biliverdin by 1·0 mmol/L potassium ferricyanide in the presence of caffeine. The absorbance decline at 480 nm with blanking at 660 nm is proportional to bilirubin concentration. The assay is linear up to 1000μmol/L and is suitable for the measurement of bilirubin in adult and neonatal patients. The assay is accurate and precise and is not significantly affected by haemolysis up to 5·0 g/L haemoglobin. Reagents and calibration are stable for at least 3 months. </jats:p>
  • Zugangsstatus: Freier Zugang