Colonization of Phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with Leishmania major and saliva
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Titel:
Colonization of Phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with Leishmania major and saliva
Beschreibung:
AbstractBackgroundSand fly saliva has been postulated as a potential vaccine or as a vaccine component within multi component vaccine against leishmaniasis. It is important to note that these studies were performed using long-term colonizedPhlebotomus papatasi. The effect of sand flies colonization on the outcome ofLeishmaniainfection is reported.ResultsWhile pre-immunization of mice with salivary gland homogenate (SGH) of long-term colonized (F5 and beyond) femalePhlebotomus papatasiinduced protection againstLeishmania majorco-inoculated with the same type of SGH, pre-immunization of mice with SGH of recently colonized (F2 and F3) femaleP. papatasidid not confer protection againstL. majorco-inoculated with the same type of SGH. Our data showed for the first time that a shift from lack of protection to protection occurs at the fourth generation (F4) during the colonization process ofP. papatasi.ConclusionFor the development of a sand fly saliva-based vaccine, inferences based on long-term colonized populations of sand flies should be treated with caution as colonization ofP. papatasiappears to modulate the outcome ofL. majorinfection from lack of protection to protection.