DRR Dhan 58, a Seedling Stage Salinity Tolerant NIL of Improved Samba Mahsuri Shows Superior Performance in Multi-location Trials

Medientyp: E-Artikel
Titel: DRR Dhan 58, a Seedling Stage Salinity Tolerant NIL of Improved Samba Mahsuri Shows Superior Performance in Multi-location Trials
Beteiligte: Rekha, G.; Abhilash Kumar, V.; Gokulan, C. G.; Koushik, M. B. V. N.; Laxmi Prasanna, B.; Kulkarni, Swapnil; Aleena, D.; Harika, G.; Hajira, S. K.; Pranathi, K.; Punniakoti, E.; Kale, R. R.; Dilip Kumar, T.; Ayyappa, D.; Anila, M.; Sinha, Pragya; Manohara, K. K.; Padmavathi, G.; Subba Rao, L. V.; Laha, G. S.; Srinivas Prasad, M. S.; Fiyaz, R. A.; Suneetha, K.; Balachandran, S. M.; [...]
Erschienen: Springer Science and Business Media LLC, 2022
Erschienen in: Rice
Sprache: Englisch
DOI: 10.1186/s12284-022-00591-3
ISSN: 1939-8425; 1939-8433
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Beschreibung: <jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Improved Samba Mahsuri (ISM) is an elite, high-yielding, bacterial blight resistant, fine-grained rice variety with low glycaemic index. It is highly sensitive to salt stress, particularly at seedling stage, which significantly reduces its yield potential in coastal areas. A salinity tolerant QTL<jats:italic>, Saltol,</jats:italic> associated with seedling stage tolerance was previously mapped on chromosome 1 (10.6–11.5 Mb) from the Indian landrace, Pokkali and is effective in different genetic backgrounds. The objective of this study was to enhance salinity tolerance of ISM by incorporating the <jats:italic>Saltol</jats:italic> QTL through marker-assisted backcross breeding using the breeding line, FL478 (Pokkali/IR29).</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>Foreground selection was carried out at each generation using five <jats:italic>Saltol-</jats:italic>specific markers and three bacterial blight resistance genes, <jats:italic>Xa21, xa13</jats:italic> and <jats:italic>xa5</jats:italic>. Background selection was conducted using 66 well distributed polymorphic SSR markers and at the BC<jats:sub>3</jats:sub>F<jats:sub>2</jats:sub> generation, a single plant with maximum recurrent parent genome recovery (95.3%) was identified and advanced to the BC<jats:sub>3</jats:sub>F<jats:sub>4</jats:sub> generation. Based on bacterial blight resistance, seedling stage salinity tolerance and resemblance to ISM, four advanced breeding lines were selected for testing in replicated experiments near Hyderabad, India. A promising near-isogenic line, DRR Dhan 58, was evaluated in multi-location trials-coastal salinity and it showed significant salinity tolerance, resistance to bacterial blight disease, high yield and excellent grain quality during the 2019 and 2020 trials. DRR Dhan 58 was 95.1% similar to ISM based on genotyping with the 90 K SNP chip. Whole genome resequencing analysis of Pokkali and FL478 which were salinity tolerant checks, ISM and DRR Dhan 58 showed a high degree of relatedness with respect to the candidate gene loci for <jats:italic>Saltol</jats:italic> and <jats:italic>OsSKC1</jats:italic> (<jats:italic>Shoot K</jats:italic><jats:sup>+</jats:sup><jats:italic>Concentration 1</jats:italic>).</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>DRR Dhan 58, possessing <jats:italic>Saltol</jats:italic> and three bacterial blight resistance genes (<jats:italic>Xa21, xa13</jats:italic> and <jats:italic>xa5</jats:italic>) in the genetic background of the Indian mega-variety of rice, Samba Mahsuri, was developed for potential cultivation in areas prone to seedling stage salinity, as well as areas with endemic bacterial blight disease. This entry had a 24% yield advantage over the recurrent parent ISM under coastal saline conditions in multi-location trials and was recently released for commercial cultivation in India.</jats:p> </jats:sec>
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