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McLellan, Jade E.; Pitcher, Ashleigh J.; Ballard, Susan A.; Grabsch, Elizabeth A.; Bell, Jan M.; Barton, Mary; Grayson, M. Lindsay

Superbugs in the supermarket? Assessing the rate of contamination with third-generation cephalosporin-resistant gram-negative bacteria in fresh Australian pork and chicken

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  • Medientyp: E-Artikel
  • Titel: Superbugs in the supermarket? Assessing the rate of contamination with third-generation cephalosporin-resistant gram-negative bacteria in fresh Australian pork and chicken
  • Beteiligte: McLellan, Jade E.; Pitcher, Ashleigh J.; Ballard, Susan A.; Grabsch, Elizabeth A.; Bell, Jan M.; Barton, Mary; Grayson, M. Lindsay
  • Erschienen: Springer Science and Business Media LLC, 2018
  • Erschienen in: Antimicrobial Resistance & Infection Control
  • Sprache: Englisch
  • DOI: 10.1186/s13756-018-0322-4
  • ISSN: 2047-2994
  • Schlagwörter: Pharmacology (medical) ; Infectious Diseases ; Microbiology (medical) ; Public Health, Environmental and Occupational Health
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  • Beschreibung: <jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Antibiotic misuse in food-producing animals is potentially associated with human acquisition of multidrug-resistant (MDR; resistance to ≥ 3 drug classes) bacteria via the food chain. We aimed to determine if MDR Gram-negative (GNB) organisms are present in fresh Australian chicken and pork products.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>We sampled raw, chicken drumsticks (CD) and pork ribs (PR) from 30 local supermarkets/butchers across Melbourne on two occasions. Specimens were sub-cultured onto selective media for third-generation cephalosporin-resistant (3GCR) GNBs, with species identification and antibiotic susceptibility determined for all unique colonies. Isolates were assessed by PCR for SHV, TEM, CTX-M, AmpC and carbapenemase genes (encoding IMP, VIM, KPC, OXA-48, NDM).</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>From 120 specimens (60 CD, 60 PR), 112 (93%) grew a 3GCR-GNB (<jats:italic>n</jats:italic> = 164 isolates; 86 CD, 78 PR); common species were <jats:italic>Acinetobacter baumannii</jats:italic> (37%), <jats:italic>Pseudomonas aeruginosa</jats:italic> (13%) and <jats:italic>Serratia fonticola</jats:italic> (12%), but only one <jats:italic>E. coli</jats:italic> isolate. Fifty-nine (36%) had evidence of 3GCR alone, 93/163 (57%) displayed 3GCR plus resistance to one additional antibiotic class, and 9/163 (6%) were 3GCR plus resistance to two additional classes. Of 158 DNA specimens, all were negative for ESBL/carbapenemase genes, except 23 (15%) which were positive for AmpC, with 22/23 considered to be inherently chromosomal, but the sole <jats:italic>E. coli</jats:italic> isolate contained a plasmid-mediated CMY-2 AmpC.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusions</jats:title> <jats:p>We found low rates of MDR-GNBs in Australian chicken and pork meat, but potential 3GCR-GNBs are common (93% specimens). Testing programs that only assess for <jats:italic>E. coli</jats:italic> are likely to severely underestimate the diversity of 3GCR organisms in fresh meat.</jats:p> </jats:sec>
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