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Li, Rong; Duan, Wenjie; Feng, Tingle; Gu, Chenyang; Zhang, Qiankun; Long, Jun; Huang, Shiying; Chen, Lukui

Lycium barbarum polysaccharide inhibits ischemia-induced autophagy by promoting the biogenesis of neural stem cells-derived extracellular vesicles to enhance the delivery of miR-133a-3p

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  • Medientyp: E-Artikel
  • Titel: Lycium barbarum polysaccharide inhibits ischemia-induced autophagy by promoting the biogenesis of neural stem cells-derived extracellular vesicles to enhance the delivery of miR-133a-3p
  • Beteiligte: Li, Rong; Duan, Wenjie; Feng, Tingle; Gu, Chenyang; Zhang, Qiankun; Long, Jun; Huang, Shiying; Chen, Lukui
  • Erschienen: Springer Science and Business Media LLC, 2023
  • Erschienen in: Chinese Medicine
  • Sprache: Englisch
  • DOI: 10.1186/s13020-023-00831-8
  • ISSN: 1749-8546
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Neural stem cell-derived extracellular vesicles (NSC-EVs) mediated endogenous neurogenesis determines a crucial impact on spontaneous recovery after stroke. Here, we checked the influence of Lycium barbarum polysaccharide (LBP) on the biogenesis of NSC-EVs and then focused on studying mechanisms of LBP in ameliorating ischemic stroke outcome.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>LBP was prepared to precondition NSCs and isolate EVs. MCAO models and primary NSCs were administrated to evaluate the therapeutic effect. RT-PCR, western blot, flow cytometry, and immunofluorescence techniques were performed to explore the mechanism.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>LBP pretreatment increased the production of NSC-EVs and improved the neuroprotective and recovery effects of NSC-EV in ischemic stroke mice. LBP-pretreated NSC-EV in a dose-dependent manner substantially reduced neuronal death compared with NSC-EV. Screening of the signaling cascade involved in the interaction between NSC-EV and neurons revealed that AMPK/mTOR signaling pathway inhibited autophagic activity in neurons receiving either treatment paradigm. NSC-EVs but not EVs collected from NSCs pretreated with the anti-miR-133a-3p oligonucleotide reduced cell death, whereas the anti-oligonucleotide promoted autophagy activity and cell death by modulating AMPK/mTOR signaling in OGD-induced primary neurons.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>LBP activated AMPK/mTOR signaling pathway by increasing the enrichment and transfer of miR-133a-3p in NSC-EVs to inhibit stroke-induced autophagy activity.</jats:p> </jats:sec>
  • Zugangsstatus: Freier Zugang