Beschreibung:
<jats:title>Abstract</jats:title><jats:sec><jats:title>Introduction</jats:title><jats:p>Periprosthetic fibroblast-like cells (PPFs) play an important role in aseptic loosening of arthroplasties. Various studies have examined PPF behavior in monolayer culture systems. However, the periprosthetic tissue is a three-dimensional (3D) mesh, which allows the cells to interact in a multidirectional way. The expression of bone remodeling markers of fibroblast-like cells in a multilayer environment changes significantly versus monolayer cultures without the addition of particles or cytokine stimulation. Gene expression of bone remodeling markers was therefore compared in fibroblast-like cells from different origins and dermal fibroblasts under transwell culture conditions versus monolayer cultures.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>PPFs from periprosthetic tissues (<jats:italic>n</jats:italic> = 12), osteoarthritic (OA) synovial fibroblast-like cells (SFs) (<jats:italic>n</jats:italic> = 6), and dermal fibroblasts (DFs) were cultured in monolayer (density 5.5 × 10<jats:sup>3</jats:sup>/cm<jats:sup>2</jats:sup>) or multilayer cultures (density 8.5 × 10<jats:sup>5</jats:sup>/cm<jats:sup>2</jats:sup>) for 10 or 21 days. Cultures were examined via histology, TRAP staining, immunohistochemistry (anti-S100a4), and quantitative real-time PCR.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Fibroblast-like cells (PPFs/SFs) and dermal fibroblasts significantly increased the expression of RANKL and significantly decreased the expression of ALP, COL1A1, and OPG in multilayer cultures. PPFs and SFs in multilayer cultures further showed a higher expression of cathepsin K, MMP-13, and TNF-α. In multilayer PPF cultures, the mRNA level of TRAP was also found to be significantly increased.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>The multilayer cultures are able to induce significant expression changes in fibroblast-like cells depending on the nature of cellular origin without the addition of any further stimulus. This system might be a useful tool to get more in vivo like results regarding fibroblast-like cell cultures.</jats:p></jats:sec>