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Medientyp:
E-Artikel
Titel:
Roles for phagocytic cells and complement in controlling relapsing fever infection
Beteiligte:
Woodman, Michael E;
Cooley, Anne E;
Avdiushko, Rita;
Bowman, Amy;
Botto, Marina;
Wooten, R Mark;
van Rooijen, Nico;
Cohen, Donald A;
Stevenson, Brian
Erschienen:
Oxford University Press (OUP), 2009
Erschienen in:
Journal of Leukocyte Biology, 86 (2009) 3, Seite 727-736
Sprache:
Englisch
DOI:
10.1189/jlb.0309169
ISSN:
1938-3673;
0741-5400
Entstehung:
Anmerkungen:
Beschreibung:
Abstract Macrophages and/or dendritic cells were found to play significant roles in both antibody-dependent and -independent control of B. hermsii infection, while bacterial binding of host factor H was found to be nonessential. Relapsing fever spirochetes, such as Borrelia hermsii, proliferate to high levels in their hosts’ bloodstream until production of IgM against borrelial surface proteins promotes bacterial clearance. The mechanisms by which B. hermsii survives in host blood, as well as the immune mediators that control this infection, remain largely unknown. It has been hypothesized that B. hermsii is naturally resistant to killing by the alternative pathway of complement activation as a result of its ability to bind factor H, a host complement regulator. However, we found that Cfh−/− mice were infected to levels identical to those seen in wild-type mice. Moreover, only a small minority of B. hermsii in the blood of wild-type mice had detectable levels of factor H adhered to their outer surfaces. In vitro, complement was found to play a statistically significant role in antibody-mediated inactivation of B. hermsii, although in vivo studies indicated that complement is not essential for host control of B. hermsii. Depletion of mφ and DC from mice had significant impacts on B. hermsii infection, and depleted mice were unable to control bloodstream infections, leading to death. Infection studies using muMT indicated a significant antibody-independent role for mφ and/or DC in host control of relapsing fever infection. Together, these findings indicate mφ and/or DC play a critical role in the production of B. hermsii-specific IgM and for antibody-independent control of spirochete levels.