> Detailanzeige

ZDANOV, STEPHANIE; DEBACQ‐CHAINIAUX, FLORENCE; TOUSSAINT, OLIVIER

Knocking Down p53 with siRNA Does Not Affect the Overexpression of p21WAF‐1 after Exposure of IMR‐90 hTERT Fibroblasts to a Sublethal Concentration of H2O2 Leading to Premature Senescence

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Knocking Down p53 with siRNA Does Not Affect the Overexpression of p21WAF‐1 after Exposure of IMR‐90 hTERT Fibroblasts to a Sublethal Concentration of H2O2 Leading to Premature Senescence
  • Beteiligte: ZDANOV, STEPHANIE; DEBACQ‐CHAINIAUX, FLORENCE; TOUSSAINT, OLIVIER
  • Erschienen: Wiley, 2007
  • Erschienen in: Annals of the New York Academy of Sciences
  • Sprache: Englisch
  • DOI: 10.1196/annals.1395.034
  • ISSN: 0077-8923; 1749-6632
  • Schlagwörter: History and Philosophy of Science ; General Biochemistry, Genetics and Molecular Biology ; General Neuroscience
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p><jats:bold><jats:sc>Abstract</jats:sc>: </jats:bold> <jats:bold>Premature senescence of IMR‐90 human diploid fibroblasts (HDFs) expressing telomerase was induced by exposure to sublethal concentration of H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>, with appearance of several biomarkers of cellular senescence like enlarged cell shape, senescence‐associated β‐galactosidase (SA ß‐gal) activity, and cell cycle arrest. The induction of stress‐induced premature senescence (SIPS) was associated with a transient increase in DNA‐binding activity of p53 and an increased expression of p21<jats:sup>WAF‐1</jats:sup>. p53 small interferent RNA (siRNA) affected the basal level of p21<jats:sup>WAF‐1</jats:sup> mRNA but did not affect the overexpression of p21<jats:sup>WAF‐1</jats:sup> after stress. This siRNA approach confirms previous results obtained with other methods.</jats:bold> </jats:p>