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VON GALL, CHARLOTTE; WEAVER, DAVID R.; MOEK, JULIANE; JILG, ANTJE; STEHLE, JÖRG H.; KORF, HORST‐WERNER

Melatonin Plays a Crucial Role in the Regulation of Rhythmic Clock Gene Expression in the Mouse Pars Tuberalis

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  • Medientyp: E-Artikel
  • Titel: Melatonin Plays a Crucial Role in the Regulation of Rhythmic Clock Gene Expression in the Mouse Pars Tuberalis
  • Beteiligte: VON GALL, CHARLOTTE; WEAVER, DAVID R.; MOEK, JULIANE; JILG, ANTJE; STEHLE, JÖRG H.; KORF, HORST‐WERNER
  • Erschienen: Wiley, 2005
  • Erschienen in: Annals of the New York Academy of Sciences
  • Sprache: Englisch
  • DOI: 10.1196/annals.1327.105
  • ISSN: 0077-8923; 1749-6632
  • Entstehung:
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  • Beschreibung: <jats:p><jats:bold>A<jats:sc>bstract</jats:sc>: </jats:bold> Circadian rhythms in physiology and behavior are driven by a central clock residing within the hypothalamic suprachiasmatic nucleus (SCN). Molecularly, the biological clock is based on the transcriptional/translational feedback loop of clock genes (<jats:italic>mPer</jats:italic>, <jats:italic>mCry</jats:italic>, <jats:italic>Clock</jats:italic>, and <jats:italic>Bmal1</jats:italic>). Circadian expression of clock genes is not limited to the SCN, but is found in many peripheral tissues. Peripheral rhythms depend on neuroendocrine/neuronal output from the SCN. Melatonin, the hormone of darkness, represents an important neuroendocrine output of the circadian clock. The hypophyseal pars tuberalis (PT) is one of the main target regions for melatonin. The aim of the study was to test whether <jats:italic>mPer</jats:italic>, <jats:italic>mCry</jats:italic>, <jats:italic>Clock</jats:italic>, and <jats:italic>Bmal1</jats:italic> are rhythmically expressed in the mouse PT and how the absence of melatonin receptors affects clock gene expression. We analyzed clock gene expression by <jats:italic>in situ</jats:italic> hybridization and compared wild‐type (WT), melatonin 1 receptor knockout (MT1 ko), and melatonin 2 receptor knockout (MT2 ko) mice. <jats:italic>mPer1</jats:italic>, <jats:italic>mCry1</jats:italic>, <jats:italic>Clock</jats:italic>, and <jats:italic>Bmal1</jats:italic>, but not <jats:italic>mPer2</jats:italic> and <jats:italic>mCry2</jats:italic>, were rhythmically expressed in the PT of WT and MT2 ko mice. In the PT of MT1 ko mice, expression of <jats:italic>mPer1</jats:italic>, <jats:italic>mCry1</jats:italic>, <jats:italic>Clock</jats:italic>, and <jats:italic>Bmal1</jats:italic> was dramatically reduced. We conclude that melatonin, acting through the MT1 receptor, is an important regulator of rhythmic clock gene expression in the mouse PT.</jats:p>