Erschienen in:
The Scientific World Journal, 2012 (2012), Seite 1-11
Sprache:
Englisch
DOI:
10.1100/2012/621969
ISSN:
1537-744X
Entstehung:
Anmerkungen:
Beschreibung:
Tumour necrosis factor-α, interferon-γand interleukin-4 are critical cytokines in regulating the immune responses against infections and tumours. In this study, we investigated the effects of three cytokines on CD40 expression in Myb-transformed hematological cells and their regulatory roles in promoting these cells into dendritic cells. We observed that both interleukin-4 and interferon-γincreased CD40 expression in these hematological cells in a dose-dependent manner, although the concentration required for interleukin-4 was significantly higher than that for interferon-γ. We found that tumour necrosis factor-αpromoted CD40 expression induced by interferon-γ, but not by interleukin-4. Our data showed that tumour necrosis factor-αplus interferon-γ-treated Myb-transformed hematological cells had the greatest ability to take up and process the model antigen DQ-Ovalbumin. Tumour necrosis factor-αalso increased the ability of interferon-γto produce the mixed lymphocyte reaction to allogenic T cells. Furthermore, only cotreatment with tumour necrosis factor-αand interferon-γinduced Myb-transformed hematological cells to express interleukin-6. These results suggest that tumour necrosis factor-αplays a key regulatory role in the development of dendritic cells from hematological progenitor cells induced by interferon-γ.