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Medientyp:
E-Artikel
Titel:
Interplay between intrinsic and acquired resistance to quinolones in Stenotrophomonas maltophilia
Beteiligte:
García‐León, Guillermo;
Salgado, Fabiola;
Oliveros, Juan Carlos;
Sánchez, María Blanca;
Martínez, José Luis
Erschienen:
Wiley, 2014
Erschienen in:Environmental Microbiology
Sprache:
Englisch
DOI:
10.1111/1462-2920.12408
ISSN:
1462-2912;
1462-2920
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Summary</jats:title><jats:p>To analyse whether the mutation‐driven resistance‐acquisition potential of a given bacterium might be a function of its intrinsic resistome, quinolones were used as selective agents and <jats:styled-content style="fixed-case"><jats:italic>S</jats:italic></jats:styled-content><jats:italic>tenotrophomonas maltophilia</jats:italic> was chosen as a bacterial model. <jats:styled-content style="fixed-case"><jats:italic>S</jats:italic></jats:styled-content><jats:italic>. maltophilia</jats:italic> has two elements – SmQnr and <jats:styled-content style="fixed-case">SmeDEF</jats:styled-content> – that are important in intrinsic resistance to quinolones. Using a battery of mutants in which either or both of these elements had been removed, the apparent mutation frequency for quinolone resistance and the phenotype of the selected mutants were found to be related to the intrinsic resistome and also depended on the concentration of the selector. Most mutants had phenotypes compatible with the overexpression of multidrug efflux pump(s); <jats:styled-content style="fixed-case">SmeDEF</jats:styled-content> overexpression was the most common cause of quinolone resistance. Whole genome sequencing showed that mutations of the <jats:styled-content style="fixed-case">SmeRv</jats:styled-content> regulator, which result in the overexpression of the efflux pump <jats:styled-content style="fixed-case">SmeVWX</jats:styled-content>, are the cause of quinolone resistance in mutants not overexpressing <jats:styled-content style="fixed-case">SmeDEF</jats:styled-content>. These results indicate that the development of mutation‐driven antibiotic resistance is highly dependent on the intrinsic resistome, which, at least for synthetic antibiotics such as quinolones, did not develop as a response to the presence of antibiotics in the natural ecosystems in which <jats:styled-content style="fixed-case"><jats:italic>S</jats:italic></jats:styled-content><jats:italic>. maltophilia</jats:italic> evolved.</jats:p>