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Hilker, Rolf; Munder, Antje; Klockgether, Jens; Losada, Patricia Moran; Chouvarine, Philippe; Cramer, Nina; Davenport, Colin F.; Dethlefsen, Sarah; Fischer, Sebastian; Peng, Huiming; Schönfelder, Torben; Türk, Oliver; Wiehlmann, Lutz; Wölbeling, Florian; Gulbins, Erich; Goesmann, Alexander; Tümmler, Burkhard

Interclonal gradient of virulence in the Pseudomonas aeruginosa pangenome from disease and environment

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  • Medientyp: E-Artikel
  • Titel: Interclonal gradient of virulence in the Pseudomonas aeruginosa pangenome from disease and environment
  • Beteiligte: Hilker, Rolf; Munder, Antje; Klockgether, Jens; Losada, Patricia Moran; Chouvarine, Philippe; Cramer, Nina; Davenport, Colin F.; Dethlefsen, Sarah; Fischer, Sebastian; Peng, Huiming; Schönfelder, Torben; Türk, Oliver; Wiehlmann, Lutz; Wölbeling, Florian; Gulbins, Erich; Goesmann, Alexander; Tümmler, Burkhard
  • Erschienen: Wiley, 2015
  • Erschienen in: Environmental Microbiology
  • Sprache: Englisch
  • DOI: 10.1111/1462-2920.12606
  • ISSN: 1462-2920; 1462-2912
  • Schlagwörter: Ecology, Evolution, Behavior and Systematics ; Microbiology
  • Entstehung:
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  • Beschreibung: <jats:title>Summary</jats:title><jats:p>The population genomics of <jats:styled-content style="fixed-case"><jats:italic>P</jats:italic></jats:styled-content><jats:italic>seudomonas aeruginosa</jats:italic> was analysed by genome sequencing of representative strains of the 15 most frequent clonal complexes in the <jats:styled-content style="fixed-case"><jats:italic>P</jats:italic></jats:styled-content><jats:italic>. aeruginosa</jats:italic> population and of the five most common clones from the environment of which so far no isolate from a human infection has been detected. Gene annotation identified 5892–7187 open reading frame (<jats:styled-content style="fixed-case">ORFs</jats:styled-content>; median 6381 <jats:styled-content style="fixed-case">ORFs</jats:styled-content>) in the 20 6.4–7.4 <jats:styled-content style="fixed-case">M</jats:styled-content>bp large genomes. The <jats:styled-content style="fixed-case"><jats:italic>P</jats:italic></jats:styled-content><jats:italic>. aeruginosa</jats:italic> pangenome consists of a conserved core of at least 4000 genes, a combinatorial accessory genome of a further 10 000 genes and 30 000 or more rare genes that are present in only a few strains or clonal complexes. Whole genome comparisons of single nucleotide polymorphism synteny indicated unrestricted gene flow between clonal complexes by recombination. Using standardized acute lettuce, <jats:styled-content style="fixed-case"><jats:italic>G</jats:italic></jats:styled-content><jats:italic>alleria mellonella</jats:italic> and murine airway infection models the full spectrum of possible host responses to <jats:styled-content style="fixed-case"><jats:italic>P</jats:italic></jats:styled-content><jats:italic>. aeruginosa</jats:italic> was observed with the 20 strains ranging from unimpaired health following infection to 100% lethality. Genome comparisons indicate that the differential genetic repertoire of clones maintains a habitat‐independent gradient of virulence in the <jats:styled-content style="fixed-case"><jats:italic>P</jats:italic></jats:styled-content><jats:italic>. aeruginosa</jats:italic> population.</jats:p>