Beschreibung:
<jats:title>Abstract</jats:title><jats:p>For membrane‐bound intracellular pathogens, the surrounding vacuole is the portal of communication with the host cell. The parasitophorous vacuole (<jats:styled-content style="fixed-case">PV</jats:styled-content>) harboring intrahepatocytic <jats:italic>Plasmodium</jats:italic> parasites satisfies the parasites' needs of nutrition and protection from host defenses to allow the rapid parasite growth that occurs during the liver stage of infection. In this study, we visualized the <jats:styled-content style="fixed-case">PV</jats:styled-content> membrane (<jats:styled-content style="fixed-case">PVM</jats:styled-content>) and the associated tubovesicular network (<jats:styled-content style="fixed-case">TVN</jats:styled-content>) through fluorescent tagging of two <jats:styled-content style="fixed-case">PVM</jats:styled-content>‐resident <jats:italic>Plasmodium berghei</jats:italic> proteins, <jats:styled-content style="fixed-case">UIS4</jats:styled-content> and <jats:styled-content style="fixed-case">IBIS1</jats:styled-content>. This strategy revealed previously unrecognized dynamics with which these membranes extend throughout the host cell. We observed dynamic vesicles, elongated clusters of membranes and long tubules that rapidly extend and contract from the <jats:styled-content style="fixed-case">PVM</jats:styled-content> in a microtubule‐dependent manner. Live microscopy, correlative light‐electron microscopy and fluorescent recovery after photobleaching enabled a detailed characterization of these membranous features, including velocities, the distribution of <jats:styled-content style="fixed-case">UIS4</jats:styled-content> and <jats:styled-content style="fixed-case">IBIS1</jats:styled-content>, and the connectivity of <jats:styled-content style="fixed-case">PVM</jats:styled-content> and <jats:styled-content style="fixed-case">TVN</jats:styled-content>. Labeling of host cell compartments revealed association of late endosomes and lysosomes with the elongated membrane clusters. Moreover, the signature host autophagosome protein <jats:styled-content style="fixed-case">LC3</jats:styled-content> was recruited to the <jats:styled-content style="fixed-case">PVM</jats:styled-content> and <jats:styled-content style="fixed-case">TVN</jats:styled-content> and colocalized with <jats:styled-content style="fixed-case">UIS4</jats:styled-content>. Together, our data demonstrate that the membranes surrounding intrahepatic <jats:italic>Plasmodium</jats:italic> are involved in active remodeling of host cells.</jats:p><jats:p><jats:inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/tra12151-gra-0001.gif" xlink:title="image" /></jats:p>