Beschreibung:
<jats:p>The amount of newly synthesized uracil nucleotides in mouse liver and intestine was determined by analysis of <jats:sup>15</jats:sup>N incorporation into the uracil nucleotide pool of these tissues after intraperitoneal infusion of <jats:sup>15</jats:sup>N‐labelled amino acids. The appearance of newly synthesized uracil nucleotides was linear with time, and essentially independent of the rate of infusion of <jats:sc>l</jats:sc>‐[<jats:sup>15</jats:sup>N]alanine. Varying the amino acid used in the infusion could affect the enrichment in the uracil ring nitrogens, but had no significant effect on the calculated amount of <jats:italic>de novo</jats:italic> synthesis. These results demonstrate the utility of this method in measuring <jats:italic>de novo</jats:italic> uracil nucleotide synthesis in mouse liver and intestine <jats:italic>in vivo</jats:italic>. The method should be a valuable tool in the effort to understand the regulation and pharmacological manipulation of <jats:italic>de novo</jats:italic> uracil nucleotide synthesis.</jats:p>