Beschreibung:
<jats:p>The effects of BRL 38227 and glibenclamide on intracellular calcium stores were investigated in permeabilized cultured airway smooth muscle cells of the rabbit using <jats:sup>45</jats:sup>Ca effluxes. BRL 38227 (10 μ<jats:sc>m</jats:sc>) reduced loading of the inositol 1,4,5‐trisphosphate (InsP<jats:sub>3</jats:sub>)‐sensitive intracellular store by 26.5% ± 1.0; this effect was antagonized by 1 μ<jats:sc>m</jats:sc> glibenclamide. BRL 38227 itself did not release calcium and had no effect on guanosine 5′‐0‐(3‐thiotriphosphate)‐induced calcium release. Glibenclamide (≥5 μ<jats:sc>m</jats:sc>) also reduced calcium loading of the intracellular store, and enhanced calcium release. These results suggest that BRL 38227 has a direct effect on intracellular calcium handling.</jats:p>