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Medientyp:
E-Artikel
Titel:
Molecular Size and Chain Length Distribution in Acanthamoeba Cellulose*
Beteiligte:
BLANTON, W. E.;
VILLEMEZ, C. L.
Erschienen:
Wiley, 1978
Erschienen in:The Journal of Protozoology
Sprache:
Englisch
DOI:
10.1111/j.1550-7408.1978.tb04412.x
ISSN:
0022-3921
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p><jats:bold>SYNOPSIS</jats:bold> Evidence obtained by total hydrolysis, partial acetolysis, periodate oxidation, as well as treatment with amylase, emulsin, and <jats:italic>Trichoderma viride</jats:italic>β‐(1→4)‐glucanase, verified that the alkali insoluble component of <jats:italic>Acanthamoeba castellanii</jats:italic> was pure β‐(1→4)‐glucan. The weight average chain length of the cellulose varied from DP = 3170 to DP = 4130 (mean DP = 3480) with polysaccharide obtained from seven seemingly identical cultures. Isolation of the cyst‐wall cellulose by nondegrading means indicated that alkali extraction was not depolymerizing the polysaccharide. Fractionation of cellulose obtained from a single culture produced fractions from DP = 550 to DP = 4550 (mean DP = 3280; 98.7% of the original cellulose), indicating that the cellulose is polydisperse.</jats:p>