Beschreibung:
<jats:title>Abstract</jats:title><jats:p>RNase E and its complex with other proteins (‘degradosome’) play an important role in RNA processing and decay in <jats:italic>Escherichia coli</jats:italic> and in many other bacteria. To identify the proteins which can potentially interact with this enzyme in mycobacteria, <jats:italic>Mycobacterium tuberculosis</jats:italic> H37Rv RNase E was cloned and expressed as a 6HisFLAG‐tagged fusion protein. Analysis of the mycobacterial RNase E overexpressed and purified from <jats:italic>M. bovis</jats:italic> BCG revealed the presence of GroEL and two other copurified proteins, products of the Mb1721 (inorganic polyphosphate/ATP‐NAD kinase) and Mb0825c (acetyltransferase) genes. Identical copies of these two genes can be found in <jats:italic>M. tuberculosis</jats:italic> H37Rv.</jats:p>