Induction of CCAAT/enhancer binding protein‐δ by cytokinins, but not by retinoic acid, during granulocytic differentiation of human myeloid leukaemia cells
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Titel:
Induction of CCAAT/enhancer binding protein‐δ by cytokinins, but not by retinoic acid, during granulocytic differentiation of human myeloid leukaemia cells
Beschreibung:
<jats:title>Summary</jats:title><jats:p>Cytokinins, purine derivatives that act as hormones to control many processes in plants, are very effective at inducing the granulocytic differentiation of human myeloid leukaemia cells. Isopentenyladenine (IPA), a potent cytokinin, significantly induced the expression of CCAAT/enhancer‐binding protein (C/EBP)<jats:italic>δ</jats:italic>, but not C/EBP<jats:italic>α</jats:italic> protein, whereas all‐<jats:italic>trans</jats:italic> retinoic acid, a well‐known inducer of granulocytic differentiation, induced C/EBP<jats:italic>α</jats:italic> but not C/EBP<jats:italic>δ</jats:italic> protein. Antisense oligonucleotide for C/EBP<jats:italic>δ</jats:italic>, but not C/EBP<jats:italic>α</jats:italic> or C/EBP<jats:italic>β</jats:italic>, effectively suppressed IPA‐induced differentiation, suggesting that the expression of C/EBP<jats:italic>δ</jats:italic> protein is necessary for cytokinin‐induced differentiation. Although C/EBP<jats:italic>α</jats:italic> is known to be crucial for granulocytic differentiation, the function of C/EBP<jats:italic>δ</jats:italic> has not been well documented in the regulation of haematopoiesis. The role of C/EBP<jats:italic>δ</jats:italic> in the granulocytic differentiation of myeloid leukaemia cells is discussed.</jats:p>