Beschreibung:
<jats:title>ABSTRACT</jats:title><jats:p>Reaction parameters were optimized to support polyethyleneglycol‐based and polypropyleneglycol‐based gel‐entrapped lipase‐mediated glycerolysis of butteroil to yield monoacylglycerol. Single liquid‐phase reactive mixtures contained 25% (w/w) butteroil dissolved in <jats:italic>t‐</jats:italic>butanol, to which glycerol and water was added. Optimum reaction conditions were 35–40 °C, and 5–10 mg enzyme reagent and 0.10g glycerol/mL substrate solution. Optimum acyl group:glycerol molar ratio was about 0.64. The optimum water content was dependent on glycerol content and was about 6% (w/w) of the glycerol at 0.10g glycerol/mL substrate solution. The gel‐entrapped lipases had half‐lives of about 35–46 cycles of 72 hr each.</jats:p>