The plasmid‐encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription
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Medientyp:
E-Artikel
Titel:
The plasmid‐encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription
Beteiligte:
Bölin, I.;
Wolf‐Watz, H.
Erschienen:
Wiley, 1988
Erschienen in:
Molecular Microbiology, 2 (1988) 2, Seite 237-245
Sprache:
Englisch
DOI:
10.1111/j.1365-2958.1988.tb00025.x
ISSN:
1365-2958;
0950-382X
Entstehung:
Anmerkungen:
Beschreibung:
SummaryThe basic Yop2b protein, encoded by the virulence plasmid pIBI of Yersinia pseudotuberculosis, is produced under Ca2+‐deficient conditions. A mutant deleted for the entire yopH gene, which encodes the Yop2b protein, was found to be avirulent. Virulence could be restored by trans‐complementation. The DNA‐Sequence of yopH predicted a 50 737 D polypeptide lacking a typical signal peptide. Transcription of yopH is regulated by both temperature and Ca2+‐concentration. Mutations within the region of the virulence plasmid known to be involved in regulating gene expression in response to Ca2+ abolished transcription of yopH. Other temperature‐sensitive mutations in the Ca2+‐regulatory locus showed a high level of transcription regardless of Ca2+‐concentration. These responses were similar to those of the yopE gene. The promoter region of the yopE and yopH genes were compared and four conserved motifs identified.