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Bohn, Yu‐Sing Tammy; Brandes, Gudrun; Rakhimova, Elza; Horatzek, Sonja; Salunkhe, Prabhakar; Munder, Antje; Van Barneveld, Andrea; Jordan, Doris; Bredenbruch, Florian; Häußler, Susanne; Riedel, Kathrin; Eberl, Leo; Jensen, Peter Østrup; Bjarnsholt, Thomas; Moser, Claus; Hoiby, Niels; Tümmler, Burkhard; Wiehlmann, Lutz

Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection

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  • Medientyp: E-Artikel
  • Titel: Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection
  • Beteiligte: Bohn, Yu‐Sing Tammy; Brandes, Gudrun; Rakhimova, Elza; Horatzek, Sonja; Salunkhe, Prabhakar; Munder, Antje; Van Barneveld, Andrea; Jordan, Doris; Bredenbruch, Florian; Häußler, Susanne; Riedel, Kathrin; Eberl, Leo; Jensen, Peter Østrup; Bjarnsholt, Thomas; Moser, Claus; Hoiby, Niels; Tümmler, Burkhard; Wiehlmann, Lutz
  • Erschienen: Wiley, 2009
  • Erschienen in: Molecular Microbiology
  • Sprache: Englisch
  • DOI: 10.1111/j.1365-2958.2008.06559.x
  • ISSN: 0950-382X; 1365-2958
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Summary</jats:title><jats:p>Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with <jats:italic>Pseudomonas aeruginosa.</jats:italic> Screening of a signature tagged mutagenesis library of the non‐piliated <jats:italic>P. aeruginosa</jats:italic> strain TBCF10839 uncovered that transposon inactivation of its <jats:italic>pilY1</jats:italic> gene rendered the bacterium more resistant against killing by neutrophils than the wild type and any other of the more than 3000 tested mutants. Inactivation of <jats:italic>pilY1</jats:italic> led to the loss of twitching motility in twitching‐proficient wild‐type PA14 and PAO1 strains, predisposed to autolysis and impaired the secretion of quinolones and pyocyanin, but on the other hand promoted growth in stationary phase and bacterial survival in murine airway infection models. The PilY1 population consisted of a major full‐length and a minor shorter PilY1* isoform. PilY1* was detectable in small extracellular quinolone‐positive aggregates, but not in the pilus. <jats:italic>P. aeruginosa</jats:italic> PilY1 is not an adhesin on the pilus tip, but assists in pilus biogenesis, twitching motility, secretion of secondary metabolites and in the control of cell density in the bacterial population.</jats:p>
  • Zugangsstatus: Freier Zugang