Wieczorek, Krzysztof;
Golecki, Bettina;
Gerdes, Lars;
Heinen, Petra;
Szakasits, Dagmar;
Durachko, Daniel M.;
Cosgrove, Daniel J.;
Kreil, David P.;
Puzio, Piotr S.;
Bohlmann, Holger;
Grundler, Florian M. W.
Expansins are involved in the formation of nematode‐induced syncytia in roots of Arabidopsis thaliana
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Medientyp:
E-Artikel
Titel:
Expansins are involved in the formation of nematode‐induced syncytia in roots of Arabidopsis thaliana
Beteiligte:
Wieczorek, Krzysztof;
Golecki, Bettina;
Gerdes, Lars;
Heinen, Petra;
Szakasits, Dagmar;
Durachko, Daniel M.;
Cosgrove, Daniel J.;
Kreil, David P.;
Puzio, Piotr S.;
Bohlmann, Holger;
Grundler, Florian M. W.
Erschienen:
Wiley, 2006
Erschienen in:The Plant Journal
Sprache:
Englisch
DOI:
10.1111/j.1365-313x.2006.02856.x
ISSN:
0960-7412;
1365-313X
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Summary</jats:title><jats:p>Parasitism of the cyst nematode <jats:italic>Heterodera schachtii</jats:italic> is characterized by the formation of syncytial feeding structures in the host root. Syncytia are formed by the fusion of root cells, accompanied by local cell wall degradation, fusion of protoplasts and hypertrophy. Expansins are cell wall‐loosening proteins involved in growth and cell wall disassembly. In this study, we analysed whether members of the expansin gene family are specifically and developmentally regulated during syncytium formation in the roots of <jats:italic>Arabidopsis thaliana</jats:italic>. We used PCR to screen a cDNA library of 5–7‐day‐old syncytia for expansin transcripts with primers differentiating between 26 <jats:italic>α</jats:italic>‐ and three <jats:italic>β</jats:italic>‐expansin cDNAs. <jats:italic>AtEXPA1</jats:italic>, <jats:italic>AtEXPA3</jats:italic>, <jats:italic>AtEXPA4</jats:italic>, <jats:italic>AtEXPA6</jats:italic>, <jats:italic>AtEXPA8</jats:italic>, <jats:italic>AtEXPA10</jats:italic>, <jats:italic>AtEXPA15</jats:italic>, <jats:italic>AtEXPA16</jats:italic>, <jats:italic>AtEXPA20</jats:italic> and <jats:italic>AtEXPB3</jats:italic> could be amplified from the library. In a semi‐quantitative RT‐PCR and a Genechip analysis <jats:italic>AtEXPA3</jats:italic>, <jats:italic>AtEXPA6</jats:italic>, <jats:italic>AtEXPA8</jats:italic>, <jats:italic>AtEXPA10</jats:italic> and <jats:italic>AtEXPA16</jats:italic> were found to be upregulated specifically in syncytia, but not to be transcribed in surrounding root tissue. Histological analyses were performed with the aid of promoter::GUS lines and <jats:italic>in situ</jats:italic> RT‐PCR. Results from both approaches supported the specific expression pattern. Among the specifically expressed genes, <jats:italic>AtEXPA3</jats:italic> and <jats:italic>AtEXPA16</jats:italic> turned out to be of special interest as they are shoot‐specific in uninfected plants. We conclude that syncytium formation involves the specific regulation of expansin genes, indicating that the encoded expansins take part in cell growth and cell wall disassembly during syncytium formation.</jats:p>