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Medientyp:
E-Artikel
Titel:
RNAi‐induced silencing of gene expression in strawberry fruit (Fragaria × ananassa) by agroinfiltration: a rapid assay for gene function analysis
Beschreibung:
<jats:title>Summary</jats:title><jats:p>Intron‐containing constructs encoding self‐complementary ‘hairpin’ RNA (ihpRNA) have the potential to efficiently silence genes in a range of plant species. In this study we demonstrate the silencing of a ripening‐related chalcone synthase (<jats:italic>CHS</jats:italic>) gene in strawberry fruits (<jats:italic>Fragaria</jats:italic> × <jats:italic>ananassa</jats:italic> cv. Elsanta) by a construct (ihpRNA) containing the partial sense and corresponding antisense sequences of <jats:italic>CHS</jats:italic> separated by an intron obtained from a <jats:italic>F.</jats:italic> × <jats:italic>ananassa</jats:italic> quinone oxidoreductase gene. An <jats:italic>Agrobacterium</jats:italic> strain carrying a T‐DNA expressing the ihpRNA transgene was injected with a syringe into the receptacles of growing fruits still attached to the plant about 14 days after pollination. As a consequence of the reduced levels of <jats:italic>CHS</jats:italic> mRNA and enzymatic CHS activity, the levels of anthocyanins were downregulated and precursors of the flavonoid pathway were shunted to the phenylpropanoid pathway leading to a large increases in levels of (hydroxy) cinnamoyl glucose esters. We anticipate that this technique in combination with metabolite profiling analysis will be useful for studying the function of unknown genes during the development and ripening of strawberry fruit.</jats:p>