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Endlich, Nicole; Lange, Tim; Kuhn, Jana; Klemm, Paul; Kotb, Ahmed M.; Siegerist, Florian; Kindt, Frances; Lindenmeyer, Maja T.; Cohen, Clemens D.; Kuss, Andreas W.; Nath, Neetika; Rettig, Rainer; Lendeckel, Uwe; Zimmermann, Uwe; Amann, Kerstin; Stracke, Sylvia; Endlich, Karlhans

BDNF: mRNA expression in urine cells of patients with chronic kidney disease and its role in kidney function

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  • Medientyp: E-Artikel
  • Titel: BDNF: mRNA expression in urine cells of patients with chronic kidney disease and its role in kidney function
  • Beteiligte: Endlich, Nicole; Lange, Tim; Kuhn, Jana; Klemm, Paul; Kotb, Ahmed M.; Siegerist, Florian; Kindt, Frances; Lindenmeyer, Maja T.; Cohen, Clemens D.; Kuss, Andreas W.; Nath, Neetika; Rettig, Rainer; Lendeckel, Uwe; Zimmermann, Uwe; Amann, Kerstin; Stracke, Sylvia; Endlich, Karlhans
  • Erschienen: Wiley, 2018
  • Erschienen in: Journal of Cellular and Molecular Medicine
  • Sprache: Englisch
  • DOI: 10.1111/jcmm.13762
  • ISSN: 1582-1838; 1582-4934
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>Podocyte loss and changes to the complex morphology are major causes of chronic kidney disease (CKD). As the incidence is continuously increasing over the last decades without sufficient treatment, it is important to find predicting biomarkers. Therefore, we measured urinary mRNA levels of podocyte genes <jats:italic>NPHS1, NPHS2, PODXL</jats:italic> and <jats:italic>BDNF, KIM‐1, CTSL</jats:italic> by qRT‐PCR of 120 CKD patients. We showed a strong correlation between BDNF and the kidney injury marker <jats:italic>KIM‐1</jats:italic>, which were also correlated with <jats:italic>NPHS1</jats:italic>, suggesting podocytes as a contributing source. In human biopsies, <jats:italic>BDNF</jats:italic> was localized in the cell body and major processes of podocytes. In glomeruli of diabetic nephropathy patients, we found a strong <jats:italic>BDNF</jats:italic> signal in the remaining podocytes. An inhibition of the <jats:italic>BDNF</jats:italic> receptor TrkB resulted in enhanced podocyte dedifferentiation. The knockdown of the orthologue resulted in pericardial oedema formation and lowered viability of zebrafish larvae. We found an enlarged Bowman's space, dilated glomerular capillaries, podocyte loss and an impaired glomerular filtration. We demonstrated that <jats:italic>BDNF</jats:italic> is essential for glomerular development, morphology and function and the expression of <jats:italic>BDNF</jats:italic> and <jats:italic>KIM‐1</jats:italic> is highly correlated in urine cells of CKD patients. Therefore, <jats:italic>BDNF</jats:italic> mRNA in urine cells could serve as a potential CKD biomarker.</jats:p>
  • Zugangsstatus: Freier Zugang