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Medientyp:
E-Artikel
Titel:
LL‐37 in periodontal health and disease and its susceptibility to degradation by proteinases present in gingival crevicular fluid
Beteiligte:
McCrudden, Maelíosa T. C.;
Orr, David F.;
Yu, Yang;
Coulter, Wilson A.;
Manning, Gwen;
Irwin, Chris R.;
Lundy, Fionnuala T.
Erschienen:
Wiley, 2013
Erschienen in:
Journal of Clinical Periodontology, 40 (2013) 10, Seite 933-941
Sprache:
Englisch
DOI:
10.1111/jcpe.12141
ISSN:
1600-051X;
0303-6979
Entstehung:
Anmerkungen:
Beschreibung:
AbstractAimTo determine the levels of LL‐37 in and its susceptibility to degradation by components of gingival crevicular fluid (GCF) in periodontal health and disease.Materials and MethodsLevels of LL‐37 in GCF from periodontitis patients and periodontally healthy subjects were determined by ELISA. In addition, degradation of synthetic/exogenous LL‐37 by components of GCF in the presence and absence of inhibitors was determined by matrix‐assisted laser desorption/ionization time of flight mass spectrometry.ResultsThe concentration of native LL‐37 in GCF from Porphyromonas gingivalis positive (Pg+) and P. gingivalis negative (Pg‐) sites in periodontitis patients was significantly higher than in GCF from healthy subjects. When synthetic LL‐37 was added to healthy GCF, the peptide was not degraded. Conversely, GCF from Pg+ sites rapidly degraded synthetic LL‐37 which was prevented in the presence of Arg− and Lys− gingipain inhibitors. Synthetic LL‐37 was degraded more slowly by GCF from Pg− sites.ConclusionsLL‐37 is detectable in GCF in periodontal health and disease. The rapid degradation of synthetic LL‐37 in periodontitis GCF, particularly in Pg+ sites, limits its role as a potential therapeutic in the gingival crevice. These results highlight the need to design stable peptide mimetics of LL‐37 as future therapeutics in periodontitis.