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Mancuso, Jordan E.; Ristenpart, William D.

A spike in mechanotransductive adenosine triphosphate release from red blood cells in microfluidic constrictions only occurs with rare donors

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  • Medientyp: E-Artikel
  • Titel: A spike in mechanotransductive adenosine triphosphate release from red blood cells in microfluidic constrictions only occurs with rare donors
  • Beteiligte: Mancuso, Jordan E.; Ristenpart, William D.
  • Erschienen: Wiley, 2018
  • Erschienen in: Microcirculation, 25 (2018) 3
  • Sprache: Englisch
  • DOI: 10.1111/micc.12439
  • ISSN: 1073-9688; 1549-8719
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>Wan et al (<jats:italic>Proc Natl Acad Sci USA</jats:italic>,<jats:bold> 105</jats:bold>, 2008, 16432) demonstrated that <jats:styled-content style="fixed-case">RBC</jats:styled-content>s rapidly and transiently release a spike of 300% more <jats:styled-content style="fixed-case">ATP</jats:styled-content> shortly downstream from a short microfluidic constriction where the cells experience a sudden increase in shear stress. More recent work by Cinar et al (<jats:italic>Proc Natl Acad Sci USA, </jats:italic><jats:bold>112</jats:bold>, 2015, 11783), however, yielded no evidence for a similar spike in <jats:styled-content style="fixed-case">ATP</jats:styled-content> release downstream of the constriction. Our aim was to determine whether a transient spike in mechanotransduction is the typical response of RBCs to the sudden onset of increased shear.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We investigate ATP release downstream of a microfluidic constriction for 15 participants using a luciferase‐based photoluminescent assay.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>While we observe mechanotransductive <jats:styled-content style="fixed-case">ATP</jats:styled-content> release from blood drawn from all donors, we find evidence of a spike in <jats:styled-content style="fixed-case">ATP</jats:styled-content> concentration after the microfluidic constriction for only 2 of 15 participants. No clear trends in <jats:styled-content style="fixed-case">ATP</jats:styled-content> release are found with respect to the magnitude of the applied shear stress, or to the gender, age, or physical activity (Baecke) index of the donor.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In aggregate, all data acquired to date suggest that a spike in mechanotransductive <jats:styled-content style="fixed-case">ATP</jats:styled-content> due to a suddenly applied increase in shear stress occurs in blood drawn from only 14% of the population.</jats:p></jats:sec>