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Medientyp:
E-Artikel
Titel:
Opportunities and limitations of reduced representation bisulfite sequencing in plant ecological epigenomics
Beteiligte:
Paun, Ovidiu;
Verhoeven, Koen J. F.;
Richards, Christina L.
Erschienen:
Wiley, 2019
Erschienen in:New Phytologist
Sprache:
Englisch
DOI:
10.1111/nph.15388
ISSN:
0028-646X;
1469-8137
Entstehung:
Anmerkungen:
Beschreibung:
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<jats:th>Contents</jats:th>
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<jats:td><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#nph15388-sec-1001">Summary</jats:ext-link></jats:td>
<jats:td>738</jats:td>
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<jats:td>I.</jats:td>
<jats:td><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#nph15388-sec-0002">Introduction</jats:ext-link></jats:td>
<jats:td>738</jats:td>
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<jats:td>II.</jats:td>
<jats:td><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#nph15388-sec-0003">RRBS loci as genome‐wide epigenetic markers</jats:ext-link></jats:td>
<jats:td>739</jats:td>
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<jats:td>III.</jats:td>
<jats:td><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#nph15388-sec-0004">Exploiting functional annotation of RRBS loci</jats:ext-link></jats:td>
<jats:td>739</jats:td>
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<jats:tr>
<jats:td>IV.</jats:td>
<jats:td><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#nph15388-sec-0005">Limitations of RRBS methods for nonmodel species</jats:ext-link></jats:td>
<jats:td>740</jats:td>
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<jats:td>V.</jats:td>
<jats:td><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#nph15388-sec-0006">Maximising the impact of RRBS in plants</jats:ext-link></jats:td>
<jats:td>741</jats:td>
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<jats:td>VI.</jats:td>
<jats:td><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#nph15388-sec-0007">Conclusions</jats:ext-link></jats:td>
<jats:td>741</jats:td>
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<jats:td><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#nph15388-sec-0008">Acknowledgements</jats:ext-link></jats:td>
<jats:td>741</jats:td>
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</jats:p></jats:sec><jats:sec><jats:title>Summary</jats:title><jats:p>Investigating the features and implications of epigenetic mechanisms across the breadth of organisms and ecosystems is important for understanding the ecological relevance of epigenetics. Several cost‐effective reduced representation bisulfite sequencing approaches (<jats:styled-content style="fixed-case">RRBS</jats:styled-content>) have been recently developed and applied to different organisms that lack a well annotated reference genome. These new approaches improve the assessment of epigenetic diversity in ecological settings and may provide functional insights. We assess here the opportunities and limitations of <jats:styled-content style="fixed-case">RRBS</jats:styled-content> in nonmodel plant species. Well thought out experimental designs that include complementary gene expression studies, and the improvement of genomics resources for the target group, promise to maximize the effect of future <jats:styled-content style="fixed-case">RRBS</jats:styled-content> studies.</jats:p></jats:sec>