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Koh, Eugene; Cohen, Dekel; Brandis, Alexander; Fluhr, Robert

Attenuation of cytosolic translation by RNA oxidation is involved in singlet oxygen‐mediated transcriptomic responses

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  • Medientyp: E-Artikel
  • Titel: Attenuation of cytosolic translation by RNA oxidation is involved in singlet oxygen‐mediated transcriptomic responses
  • Beteiligte: Koh, Eugene; Cohen, Dekel; Brandis, Alexander; Fluhr, Robert
  • Erschienen: Wiley, 2021
  • Erschienen in: Plant, Cell & Environment
  • Sprache: Englisch
  • DOI: 10.1111/pce.14162
  • ISSN: 0140-7791; 1365-3040
  • Entstehung:
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  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>Singlet oxygen (<jats:sup>1</jats:sup>O<jats:sub>2</jats:sub>) production is associated with stress signalling. Here, using Arabidopsis as a model system, we study the effects of the accumulation of 8‐hydroxyguanosine (8‐oxoG), a major product of <jats:sup>1</jats:sup>O<jats:sub>2</jats:sub>‐mediated RNA oxidation. We show that 8‐oxoG can accumulate in vivo when <jats:sup>1</jats:sup>O<jats:sub>2</jats:sub> is produced in the cytoplasm. Conditions for such production include the application of RB in the light, dark‐to‐light transitions in the <jats:italic>flu</jats:italic> mutant, or subjecting plants to combined dehydration/light exposure. Transcriptomes of these treatments displayed a significant overlap with transcripts stimulated by the cytosolic 80S ribosomal translation inhibitors, cycloheximide and homoharringtonine. We demonstrate that 8‐oxoG accumulation correlates with a decrease in RNA translatability, resulting in the rapid decrease of the levels of labile gene repressor elements such as IAA1 and JAZ1 in a proteasome‐dependent manner. Indeed, genes regulated by the labile repressors of the jasmonic acid signalling pathway were induced by cycloheximide, RB or dehydration/light treatment independently of the hormone. The results suggest that <jats:sup>1</jats:sup>O<jats:sub>2</jats:sub>, by oxidizing RNA, attenuated cellular translatability and caused specific genes to be released from the repression of their cognate short half‐life repressors. The findings here describe a novel means of gene regulation via the direct interaction of <jats:sup>1</jats:sup>O<jats:sub>2</jats:sub> with RNA.</jats:p>