Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Oryza sativa,C4HC3‐type really interesting new gene (RING),OsRFPv6, is a positive regulator in response to salt stress by regulating Na+absorption
Beteiligte:
Kim, Jong Ho;
Lim, Sung Don;
Jang, Cheol Seong
Erschienen:
Wiley, 2021
Erschienen in:Physiologia Plantarum
Sprache:
Englisch
DOI:
10.1111/ppl.13481
ISSN:
0031-9317;
1399-3054
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Abstract</jats:title><jats:p>Salinity negatively affects plant growth, productivity, and metabolism. Therefore, plants have evolved diverse strategies to survive in saline environments. To identify such strategies involving the ubiquitin/26S proteasome system, we characterized molecular functions of a rice C4HC3 really interesting new gene (RING)‐type E3‐ubiquitin ligase gene.<jats:italic>Oryza sativa</jats:italic>RING finger protein v6 (OsRFPv6) was highly expressed under conditions of abiotic stress, induced by 100 mM NaCl and 20% PEG. The GFP‐OsRFPv6 protein was localized in the plasma membrane and cytosol in rice protoplasts. In vitro ubiquitin assay revealed that OsRFPv6 possessed E3‐ubiquitin ligase activity, but its variant OsRFPv6C100A did not. OsRFPv6‐overexpressing plants were insensitive to salinity, but their growth was delayed under normal conditions. Under saline conditions, transgenic plants exhibited higher proline, soluble sugar, and chlorophyll content and lower H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>accumulation than wild‐type plants. Moreover, transgenic plants exhibited lower Na<jats:sup>+</jats:sup>uptake, lower Na<jats:sup>+</jats:sup>content, and higher K<jats:sup>+</jats:sup>content in the xylem sap assay. Under saline conditions, the expression levels of nine Na<jats:sup>+</jats:sup>/K<jats:sup>+</jats:sup>transporter genes in roots and leaves were significantly different between transgenic and wild‐type plants. Specifically, under both normal and saline conditions, the expression of OsHKT2;1, a Na<jats:sup>+</jats:sup>transporter, in the roots of transgenic plants was lower than that in the roots of wild‐type plants. These results suggest that OsRFPv6 E3‐ubiquitin ligase serves as a positive regulator of salinity response via Na<jats:sup>+</jats:sup>uptake.</jats:p>