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Medientyp:
E-Artikel
Titel:
An Alternative Method to Characterize the Quasi-Static, Nonlinear Material Properties of Murine Articular Cartilage
Beteiligte:
Kotelsky, Alexander;
Woo, Chandler W.;
Delgadillo, Luis F.;
Richards, Michael S.;
Buckley, Mark R.
Erschienen:
ASME International, 2018
Erschienen in:
Journal of Biomechanical Engineering, 140 (2018) 1
Sprache:
Englisch
DOI:
10.1115/1.4038147
ISSN:
0148-0731;
1528-8951
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>With the onset and progression of osteoarthritis (OA), articular cartilage (AC) mechanical properties are altered. These alterations can serve as an objective measure of tissue degradation. Although the mouse is a common and useful animal model for studying OA, it is extremely challenging to measure the mechanical properties of murine AC due to its small size (thickness < 50 μm). In this study, we developed novel and direct approach to independently quantify two quasi-static mechanical properties of mouse AC: the load-dependent (nonlinear) solid matrix Young's modulus (E) and drained Poisson's ratio (ν). The technique involves confocal microscope-based multiaxial strain mapping of compressed, intact murine AC followed by inverse finite element analysis (iFEA) to determine E and ν. Importantly, this approach yields estimates of E and ν that are independent of the initial guesses used for iterative optimization. As a proof of concept, mechanical properties of AC on the medial femoral condyles of wild-type mice were obtained for both trypsin-treated and control specimens. After proteolytic tissue degradation induced through trypsin treatment, a dramatic decrease in E was observed (compared to controls) at each of the three tested loading conditions. A significant decrease in ν due to trypsin digestion was also detected. These data indicate that the method developed in this study may serve as a valuable tool for comparative studies evaluating factors involved in OA pathogenesis using experimentally induced mouse OA models.</jats:p>