Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Cloning and Expression of a Rat Brain GABA Transporter
Beteiligte:
Guastella, John;
Nelson, Nathan;
Nelson, Hannah;
Czyzyk, Linda;
Keynan, Shoshi;
Miedel, May C.;
Davidson, Norman;
Lester, Henry A.;
Kanner, Baruch I.
Erschienen:
American Association for the Advancement of Science (AAAS), 1990
Erschienen in:
Science, 249 (1990) 4974, Seite 1303-1306
Sprache:
Englisch
DOI:
10.1126/science.1975955
ISSN:
0036-8075;
1095-9203
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>
A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter γ-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in
<jats:italic>Xenopus</jats:italic>
oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [
<jats:sup>3</jats:sup>
H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium-and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.
</jats:p>