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Medientyp:
E-Artikel
Titel:
Water-in-Carbon Dioxide Microemulsions: An Environment for Hydrophiles Including Proteins
Beteiligte:
Johnston, K. P.;
Harrison, K. L.;
Clarke, M. J.;
Howdle, S. M.;
Heitz, M. P.;
Bright, F. V.;
Carlier, C.;
Randolph, T. W.
Erschienen:
American Association for the Advancement of Science (AAAS), 1996
Erschienen in:
Science, 271 (1996) 5249, Seite 624-626
Sprache:
Englisch
DOI:
10.1126/science.271.5249.624
ISSN:
0036-8075;
1095-9203
Entstehung:
Anmerkungen:
Beschreibung:
Carbon dioxide in the liquid and supercritical fluid states is useful as a replacement for toxic organic solvents. However, nonvolatile hydrophilic substances such as proteins, ions, and most catalysts are insoluble. This limitation was overcome by the formation of aqueous microemulsion droplets in a carbon dioxide-continuous phase with a nontoxic ammonium carboxylate perfluoropolyether surfactant. Several spectroscopic techniques consistently indicated that the properties of the droplets approach those of bulk water. The protein bovine serum albumin (BSA) with a molecular weight of 67,000 is soluble in this microemulsion and experiences an environment similar to that of native BSA in buffer.