Komm, Barry S.;
Terpening, Christopher M.;
Benz, David J.;
Graeme, Kimberlie A.;
Gallegos, Alfred;
Korc, Murray;
Greene, Geoffrey L.;
O'Malley, Bert W.;
Haussler, Mark R.
Estrogen Binding, Receptor mRNA, and Biologic Response in Osteoblast-Like Osteosarcoma Cells
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Medientyp:
E-Artikel
Titel:
Estrogen Binding, Receptor mRNA, and Biologic Response in Osteoblast-Like Osteosarcoma Cells
Beteiligte:
Komm, Barry S.;
Terpening, Christopher M.;
Benz, David J.;
Graeme, Kimberlie A.;
Gallegos, Alfred;
Korc, Murray;
Greene, Geoffrey L.;
O'Malley, Bert W.;
Haussler, Mark R.
Erschienen:
American Association for the Advancement of Science (AAAS), 1988
Erschienen in:
Science, 241 (1988) 4861, Seite 81-84
Sprache:
Englisch
DOI:
10.1126/science.3164526
ISSN:
0036-8075;
1095-9203
Entstehung:
Anmerkungen:
Beschreibung:
High specific activity estradiol labeled with iodine-125 was used to detect approximately 200 saturable, high-affinity (dissociation constant ≅ 1.0 n M ) nuclear binding sites in rat (ROS 17/2.8) and human (HOS TE85) clonal osteoblast-like osteosarcoma cells. Of the steroids tested, only testosterone exhibited significant cross-reactivity with estrogen binding. RNA blot analysis with a complementary DNA probe to the human estrogen receptor revealed putative receptor transcripts of 6 to 6.2 kilobases in both rat and human osteosarcoma cells. Type I procollagen and transforming growth factor-β messenger RNA levels were enhanced in cultured human osteoblast-like cells treated with 1 n M estradiol. Thus, estrogen can act directly on osteoblasts by a receptor-mediated mechanism and thereby modulate the extracellular matrix and other proteins involved in the maintenance of skeletal mineralization and remodeling.