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Medientyp:
E-Artikel
Titel:
A New Resistance Gene, linB , Conferring Resistance to Lincosamides by Nucleotidylation in Enterococcus faecium HM1025
Beteiligte:
Bozdogan, Bülent;
Berrezouga, Latifa;
Kuo, Ming-Shang;
Yurek, David A.;
Farley, Kathleen A.;
Stockman, Brian J.;
Leclercq, Roland
Erschienen:
American Society for Microbiology, 1999
Erschienen in:Antimicrobial Agents and Chemotherapy
Sprache:
Englisch
DOI:
10.1128/aac.43.4.925
ISSN:
0066-4804;
1098-6596
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>ABSTRACT</jats:title>
<jats:p>
Resistance to lincomycin and clindamycin in the clinical isolate
<jats:italic>Enterococcus faecium</jats:italic>
HM1025 is due to a ribosomal methylase encoded by an
<jats:italic>ermAM</jats:italic>
-like gene and the plasmid-mediated inactivation of these antibiotics. We have cloned and determined the nucleotide sequence of the gene responsible for the inactivation of lincosamides,
<jats:italic>linB</jats:italic>
. This gene encodes a 267-amino-acid lincosamide nucleotidyltransferase. The enzyme catalyzes 3-(5′-adenylation) (the adenylation of the hydroxyl group in position 3 of the molecules) of lincomycin and clindamycin. Expression of
<jats:italic>linB</jats:italic>
was observed in both
<jats:italic>Escherichia coli</jats:italic>
and
<jats:italic>Staphylococcus aureus</jats:italic>
. The deduced amino acid sequence of the enzyme did not display any significant homology with staphylococcal nucleotidyltransferases encoded by
<jats:italic>linA</jats:italic>
and
<jats:italic>linA</jats:italic>
′ genes. Sequences homologous to
<jats:italic>linB</jats:italic>
were found in 14 other clinical isolates of
<jats:italic>E. faecium</jats:italic>
, indicating the spread of the resistance trait in this species.
</jats:p>