Beschreibung:
<jats:p>
We demonstrate that a cobalamin chemical probe can be used to investigate
<jats:italic>in vivo</jats:italic>
roles of vitamin B
<jats:sub>12</jats:sub>
in microbial growth and regulation by supporting the growth of B
<jats:sub>12</jats:sub>
auxotrophic bacteria and archaea, enabling biological activity with three different cell macromolecules (RNA, DNA, and proteins), and facilitating functional proteomics to characterize B
<jats:sub>12</jats:sub>
-protein interactions. The B
<jats:sub>12</jats:sub>
-ABP is both transcriptionally and translationally able to regulate gene expression analogous to natural vitamin B
<jats:sub>12</jats:sub>
. The application of the B
<jats:sub>12</jats:sub>
-ABP at biologically relevant concentrations facilitates a unique way to measure B
<jats:sub>12</jats:sub>
microbial dynamics and identify new B
<jats:sub>12</jats:sub>
protein targets in bacteria and archaea. We demonstrate that the B
<jats:sub>12</jats:sub>
-ABP can be used to identify
<jats:italic>in vivo</jats:italic>
protein interactions across diverse microbes, from
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
to microbes isolated from naturally occurring phototrophic biofilms to the salt-tolerant archaea
<jats:named-content content-type="genus-species">Haloferax volcanii</jats:named-content>
.
</jats:p>