Erschienen:
American Society for Microbiology, 2009
Erschienen in:
Applied and Environmental Microbiology, 75 (2009) 10, Seite 3069-3076
Sprache:
Englisch
DOI:
10.1128/aem.02864-08
ISSN:
0099-2240;
1098-5336
Entstehung:
Anmerkungen:
Beschreibung:
ABSTRACT The impacts of 12 common food industry stresses on the single-cell growth probability and single-cell lag time distribution of Listeria monocytogenes were determined in half Fraser broth, the primary enrichment broth of the International Organization for Standardization detection method. First, it was determined that the ability of a cell to multiply in half Fraser broth is conditioned by its history (the probability for a cell to multiply can be decreased to 0.05), meaning that, depending on the stress in question, the risk of false-negative samples can be very high. Second, it was established that when cells are injured, the single-cell lag times increase in mean and in variability and that this increase represents a true risk of not reaching the detection threshold of the method in the enrichment broth. No relationship was observed between the impact on single-cell lag times and that on growth probabilities. These results emphasize the importance of taking into account the physiological state of the cells when evaluating the performance of methods to detect pathogens in food.