Erschienen:
American Society for Microbiology, 2018
Erschienen in:Applied and Environmental Microbiology
Sprache:
Englisch
DOI:
10.1128/aem.02335-17
ISSN:
0099-2240;
1098-5336
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>ABSTRACT</jats:title>
<jats:p>
Recent declines in bumble bee populations are of great concern and have prompted critical evaluations of the role of pathogen introductions and host resistance in bee health. One factor that may influence host resilience when facing infection is the gut microbiota. Previous experiments with
<jats:named-content content-type="genus-species">Bombus terrestris</jats:named-content>
, a European bumble bee, showed that the gut microbiota can protect against
<jats:named-content content-type="genus-species">Crithidia bombi</jats:named-content>
, a widespread trypanosomatid parasite of bumble bees. However, the particular characteristics of the microbiome responsible for this protective effect have thus far eluded identification. Using wild and commercially sourced
<jats:named-content content-type="genus-species">Bombus impatiens</jats:named-content>
, an important North American pollinator, we conducted cross-wise microbiota transplants to naive hosts of both backgrounds and challenged them with a
<jats:named-content content-type="genus-species">Crithidia</jats:named-content>
parasite. As with
<jats:named-content content-type="genus-species">B. terrestris</jats:named-content>
, we find that microbiota-dependent protection against
<jats:named-content content-type="genus-species">Crithidia</jats:named-content>
operates in
<jats:named-content content-type="genus-species">B. impatiens</jats:named-content>
. Lower
<jats:named-content content-type="genus-species">Crithidia</jats:named-content>
infection loads were experimentally associated with high microbiome diversity, large gut bacterial populations, and the presence of
<jats:named-content content-type="genus-species">Apibacter</jats:named-content>
,
<jats:named-content content-type="genus-species">Lactobacillus</jats:named-content>
Firm-5, and
<jats:named-content content-type="genus-species">Gilliamella</jats:named-content>
spp. in the gut community. These results indicate that even subtle differences between gut community structures can have a significant impact on a microbiome's ability to defend against parasite infections.
</jats:p>
<jats:p>
<jats:bold>IMPORTANCE</jats:bold>
Many wild bumble bee populations are under threat due to human activity, including through the introduction of pathogens via commercially raised bees. Recently, it was found that the bumble bee gut microbiota can help defend against a common parasite,
<jats:named-content content-type="genus-species">Crithidia bombi</jats:named-content>
, but the particular factors contributing to this protection are unknown. Using both wild and commercially raised bees, we conducted microbiota transplants to show that microbiome diversity, total gut bacterial load, and the presence of certain core members of the microbiota may all impact bee susceptibility to
<jats:named-content content-type="genus-species">Crithidia</jats:named-content>
infection. Bee origin (genetic background) was also a factor. Finally, by examining this phenomenon in a previously uninvestigated bee species, our study demonstrates that microbiome-mediated resistance to
<jats:named-content content-type="genus-species">Crithidia</jats:named-content>
is conserved across multiple bumble bee species. These findings highlight how intricate interactions between hosts, microbiomes, and parasites can have wide-ranging consequences for the health of ecologically important species.
</jats:p>