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van Niel, Ed W. J.; Gottschal, Jan C.

Oxygen Consumption byDesulfovibrioStrains with and without Polyglucose

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  • Medientyp: E-Artikel
  • Titel: Oxygen Consumption byDesulfovibrioStrains with and without Polyglucose
  • Beteiligte: van Niel, Ed W. J.; Gottschal, Jan C.
  • Erschienen: American Society for Microbiology, 1998
  • Erschienen in: Applied and Environmental Microbiology
  • Sprache: Englisch
  • DOI: 10.1128/aem.64.3.1034-1039.1998
  • ISSN: 1098-5336; 0099-2240
  • Entstehung:
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  • Beschreibung: <jats:title>ABSTRACT</jats:title><jats:p>The kinetics of oxygen reduction by<jats:italic>Desulfovibrio salexigens</jats:italic>Mast1 and the role of polyglucose in this activity were examined and compared with those of strains of<jats:italic>D. desulfuricans</jats:italic>and<jats:italic>D. gigas</jats:italic>. Oxidation rates were highest at air saturation (up to 40 nmol of O<jats:sub>2</jats:sub>min<jats:sup>−1</jats:sup>mg of protein<jats:sup>−1</jats:sup>) and declined with decreasing oxygen concentrations. Studies with cell extracts (CE) indicated that NADH oxidase was entirely responsible for the oxygen reduction in strain Mast1. In<jats:italic>D. desulfuricans</jats:italic>CSN, at least three independent systems appeared to reduce oxygen. Two were active at all oxygen concentrations (NADH oxidase and NADPH oxidase), and one was maximally active at less than 10 μM oxygen. In contrast to<jats:italic>D. gigas</jats:italic>and<jats:italic>D. salexigens</jats:italic>strains, the<jats:italic>D. desulfuricans</jats:italic>strains also contained NADH peroxidase and NADPH peroxidase activities and did not accumulate polyglucose under nonlimiting growth conditions. At air saturation, initial activities of the oxidases and peroxidases of cells harvested at the end of the log phase were on the order of 20 to 140 nmol of O<jats:sub>2</jats:sub>min<jats:sup>−1</jats:sup>mg of protein<jats:sup>−1</jats:sup>. In all strains, these enzymes were relatively stable but were susceptible to inactivation as soon as substrates were added to the assay mixture. Under those conditions, all oxidation activity disappeared after ca. 1 h of incubation. The same finding was observed with whole cells of<jats:italic>D. desulfuricans</jats:italic>CSN and<jats:italic>D. desulfuricans</jats:italic>ATCC 27774, but inactivation was less pronounced with cells of<jats:italic>D. salexigens</jats:italic>Mast1. It appeared that the presence of polyglucose in the whole cells retarded the process of inactivation of NADH oxidase, but this property was lost in crude CE. In spite of the effect of polyglucose on the oxidative potential, oxygen-dependent growth of<jats:italic>D. salexigens</jats:italic>Mast1 could be demonstrated neither in batch nor in continuous culture.</jats:p>
  • Zugangsstatus: Freier Zugang