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Medientyp:
E-Artikel
Titel:
High Rates of Conjugation in Bacterial Biofilms as Determined by Quantitative In Situ Analysis
Beteiligte:
Hausner, Martina;
Wuertz, Stefan
Erschienen:
American Society for Microbiology, 1999
Erschienen in:
Applied and Environmental Microbiology, 65 (1999) 8, Seite 3710-3713
Sprache:
Englisch
DOI:
10.1128/aem.65.8.3710-3713.1999
ISSN:
0099-2240;
1098-5336
Entstehung:
Anmerkungen:
Beschreibung:
ABSTRACT Quantitative in situ determination of conjugative gene transfer in defined bacterial biofilms using automated confocal laser scanning microscopy followed by three-dimensional analysis of cellular biovolumes revealed conjugation rates 1,000-fold higher than those determined by classical plating techniques. Conjugation events were not affected by nutrient concentration alone but were influenced by time and biofilm structure.