Beschreibung:
ABSTRACTThe role of CD4+T-cell interleukin-4 (IL-4) receptor alpha (IL-4Rα) expression in T helper 2 (TH2) immune responses has not been defined. To examine this role, we infected CD4+T-cell IL-4Rα knockout (KO) mice with the parasitic nematodeNippostrongylus brasiliensis, which induces strong host TH2 responses. AlthoughN. brasiliensisexpulsion was not affected in CD4+T-cell IL-4Rα KO mice, the associated lung pathology was reduced. Infected CD4+T-cell IL-4Rα KO mice showed abrogation of airway mucus production. Furthermore, CD4+T-cell IL-4Rα KO mouse lungs contained reduced numbers of lymphocytes and eosinophils. Restimulation of pulmonary region-associated T-cell populations showed that TH2 cytokine responses were disrupted. Secretion of IL-4, but not secretion of IL-13 or IL-5, from mediastinal lymph node CD4+T cells was reduced in infected CD4+T-cell IL-4Rα KO mice. Restimulation of tissue-derived CD4+T cells resulted in equivalent levels of IL-4 and IL-13 on day 7 postinfection (p.i.) in control and CD4+T-cell IL-4Rα KO mice. By day 10 p.i. the TH2 cytokine levels had significantly declined in CD4+T-cell IL-4Rα KO mice. Restimulation withN. brasiliensisantigen of total lung cell populations and populations with CD4+T cells depleted showed that CD4+T cells were a key TH2 cytokine source. These data demonstrated that CD4+T-cell IL-4 responsiveness facilitates eosinophil and lymphocyte recruitment, lymphocyte localization, and TH2 cytokine production in the allergic pathology associated withN. brasiliensisinfections.