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Weidensdorfer, Marko; Chae, Ju Ik; Makobe, Celestine; Stahl, Julia; Averhoff, Beate; Müller, Volker; Schürmann, Christoph; Brandes, Ralf P.; Wilharm, Gottfried; Ballhorn, Wibke; Christ, Sara; Linke, Dirk; Fischer, Doris; Göttig, Stephan; Kempf, Volkhard A. J.

Analysis of Endothelial Adherence of Bartonella henselae and Acinetobacter baumannii Using a Dynamic Human Ex Vivo Infection Model

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  • Medientyp: E-Artikel
  • Titel: Analysis of Endothelial Adherence of Bartonella henselae and Acinetobacter baumannii Using a Dynamic Human Ex Vivo Infection Model
  • Beteiligte: Weidensdorfer, Marko; Chae, Ju Ik; Makobe, Celestine; Stahl, Julia; Averhoff, Beate; Müller, Volker; Schürmann, Christoph; Brandes, Ralf P.; Wilharm, Gottfried; Ballhorn, Wibke; Christ, Sara; Linke, Dirk; Fischer, Doris; Göttig, Stephan; Kempf, Volkhard A. J.
  • Erschienen: American Society for Microbiology, 2016
  • Erschienen in: Infection and Immunity
  • Sprache: Englisch
  • DOI: 10.1128/iai.01502-15
  • ISSN: 0019-9567; 1098-5522
  • Schlagwörter: Infectious Diseases ; Immunology ; Microbiology ; Parasitology
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>ABSTRACT</jats:title> <jats:p> Bacterial adherence determines the virulence of many human-pathogenic bacteria. Experimental approaches elucidating this early infection event in greater detail have been performed using mainly methods of cellular microbiology. However, <jats:italic>in vitro</jats:italic> infections of cell monolayers reflect the <jats:italic>in vivo</jats:italic> situation only partially, and animal infection models are not available for many human-pathogenic bacteria. Therefore, <jats:italic>ex vivo</jats:italic> infection of human organs might represent an attractive method to overcome these limitations. We infected whole human umbilical cords <jats:italic>ex vivo</jats:italic> with <jats:named-content content-type="genus-species">Bartonella henselae</jats:named-content> or <jats:named-content content-type="genus-species">Acinetobacter baumannii</jats:named-content> under dynamic flow conditions mimicking the <jats:italic>in vivo</jats:italic> infection situation of human endothelium. For this purpose, methods for quantifying endothelium-adherent wild-type and trimeric autotransporter adhesin (TAA)-deficient bacteria were set up. Data revealed that (i) <jats:named-content content-type="genus-species">A. baumannii</jats:named-content> binds in a TAA-dependent manner to endothelial cells, (ii) this organ infection model led to highly reproducible adherence rates, and furthermore, (iii) this model allowed to dissect the biological function of TAAs in the natural course of human infections. These findings indicate that infection models using <jats:italic>ex vivo</jats:italic> human tissue samples (“organ microbiology”) might be a valuable tool in analyzing bacterial pathogenicity with the capacity to replace animal infection models at least partially. </jats:p>
  • Zugangsstatus: Freier Zugang