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Medientyp:
E-Artikel
Titel:
Role ofBordetella pertussisVirulence Factors in Adherence to Epithelial Cell Lines Derived from the Human Respiratory Tract
Beteiligte:
van den Berg, Bernard M.;
Beekhuizen, Henry;
Willems, Rob J. L.;
Mooi, Frits R.;
van Furth, Ralph
Erschienen:
American Society for Microbiology, 1999
Erschienen in:
Infection and Immunity, 67 (1999) 3, Seite 1056-1062
Sprache:
Englisch
DOI:
10.1128/iai.67.3.1056-1062.1999
ISSN:
0019-9567;
1098-5522
Entstehung:
Anmerkungen:
Beschreibung:
ABSTRACTDuring colonization of the respiratory tract byBordetella pertussis, virulence factors contribute to adherence of the bacterium to the respiratory tract epithelium. In the present study, we examined the roles of the virulence factors filamentous hemagglutinin (FHA), fimbriae, pertactin (Prn), and pertussis toxin (PT) in the adherence ofB. pertussisto cells of the human bronchial epithelial cell line NCI-H292and of the laryngeal epithelial cell line HEp-2. UsingB. pertussismutant strains and purified FHA, fimbriae, Prn, and PT, we demonstrated that both fimbriae and FHA are involved in the adhesion ofB. pertussisto laryngeal epithelial cells, whereas only FHA is involved in the adherence to bronchial epithelial cells. For PT and Prn, no role as adhesion factor was found. However, purified PT bound to both bronchial and laryngeal cells and as such reduced the adherence ofB. pertussisto these cells. These data may imply that fimbriae play a role in infection of only the laryngeal mucosa, while FHA is the major factor in colonization of the entire respiratory tract.