• Medientyp: E-Artikel
  • Titel: Detection of a Mixed Infection in a Culture-Negative Brain Abscess by Broad-Spectrum Bacterial 16S rRNA Gene PCR
  • Beteiligte: Keller, Peter M.; Rampini, Silvana K.; Bloemberg, Guido V.
  • Erschienen: American Society for Microbiology, 2010
  • Erschienen in: Journal of Clinical Microbiology, 48 (2010) 6, Seite 2250-2252
  • Sprache: Englisch
  • DOI: 10.1128/jcm.01922-09
  • ISSN: 0095-1137; 1098-660X
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  • Beschreibung: ABSTRACT We describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16S rRNA gene PCR. Simultaneous detection of Fusobacterium nucleatum and Porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16S rRNA genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis.
  • Zugangsstatus: Freier Zugang